Michihiro Kobayashi, PhD

Michihiro Kobayashi, PhD
Michihiro Kobayashi, PhD

Research Assistant Professor, Department of Investigative Medicine

Biosketch

Michihiro Kobayashi, MD, PhD received his degrees from the Mie University School of Medicine in Japan, with extensive training in Hematology/ Oncology. Dr. Kobayashi has broad and extensive experience in clinical Hematology/ Oncology and general Pediatrics. After initial training, he worked at the National Cancer Center Hospital in Tokyo, Japan. Through these experiences he has received expertise in clinical (and basic) hematopoietic cell transplantation. He is also very competent in hematopoietic stem cell biology. After serving as Assistant Professor at the Kyoto University in 2005, Dr. Kobayashi sought to continue his scientific pursuits in stem cell biology at the Indiana University School of Medicine (IUSM). The extensive trainings have enriched his knowledge in both stem cell biology and cancer biology, and further developed his expertise in analyzing stem cell behavior in genetically modified mice. Dr. Kobayashi also has experience with multiple mouse models of human acute myeloid leukemia and myeloproliferative diseases including MLL-AF9-, AML-ETO-, Notch- mutant-cKit- and Ras-induced leukemia. Dr. Kobayashi is currently focusing on embryonic hematopoiesis in collaboration with Dr Yoshimoto, who is an expert of fetal/ embryonic hematopoiesis, and he is currently investigating tissue resident innate immune cells.

  • Education and Training
    • PhD 2000, Mie University
    • MD 1990, Mie University

  • Research
    • Hematopoietic Stem Cells
    • Developmental Hematology
    • Childhood Acute Leukemia
    • Cellular Immunology

  • Publications

    • Cox G., Kobayashi M., Rudd B.D., Yoshimoto M. Regulation of HSC development and function by Lin28b Frontiers in Cell and Developmental Biology. 2025;13

    • Chen H., Bai Y., Kobayashi M., Xiao S., Barajas S., Cai W., Chen S., Miao J., Meke F.N., Yao C., Yang Y., Strube K., Satchivi O., Sun J., Ronnstrand L., Croop J.M., Boswell H.S., Jia Y., Liu H., Li L.S., Altman J.K., Eklund E.A., Sukhanova M., Ji P., Tong W., Band H., Huang D.T., Platanias L.C., Zhang Z.Y., Liu Y. Molecular Cancer Research. 2024;22:94-103.

    • Syed A., Kobayashi M., Yoshimoto M. STAR Protocols. 2024;5

    • Chen H., Bai Y., Kobayashi M., Xiao S., Cai W., Barajas S., Chen S., Miao J., Meke F.N., Vemula S., Ropa J.P., Croop J.M., Boswell H.S., Wan J., Jia Y., Liu H., Li L.S., Altman J.K., Eklund E.A., Ji P., Tong W., Band H., Huang D.T., Platanias L.C., Zhang Z.Y., Liu Y. Blood. 2023;141:244-259.

    • Kobayashi M., Wei H., Yamanashi T., Azevedo Portilho N., Cornelius S., Valiente N., Nishida C., Cheng H., Latorre A., Zheng W.J., Kang J., Seita J., Shih D.J., Wu J.Q., Yoshimoto M. Cell Reports. 2023;42(3)

    • Kobayashi M., Yoshimoto M. Immunological Reviews. 2023;315(1):11-30.

    • Banerjee P., Olmsted-Davis E.A., Deswal A., Nguyen M.T., Koutroumpakis E., Palaskas N.L., Lin S.H., Kotla S., Reyes-Gibby C., Yeung S.C.J., Yusuf S.W., Yoshimoto M., Kobayashi M., Yu B., Schadler K., Herrmann J., Cooke J.P., Jain A., Chini E., Le N.T., Abe J.I. Cancer treatment-induced NAD<sup>+</sup> depletion in premature senescence and late cardiovascular complications Journal of Cardiovascular Aging. 2022;2

    • Imanishi M., Cheng H., Kotla S., Deswal A., Le N.T., Chini E., Ko K.A., Samanthapudi V.S.K., Lee L.L., Herrmann J., Xu X., Reyes-Gibby C., Yeung S.C.J., Schadler K.L., Yusuf S.W., Liao Z., Nurieva R., Amir E.a.D., Burks J.K., Palaskas N.L., Cooke J.P., Lin S.H., Kobayashi M., Yoshimoto M., Abe J.I. Radiation therapy induces immunosenescence mediated by p90RSK Frontiers in Cardiovascular Medicine. 2022;9

    • Yoshimoto M., Kosters A., Cornelius S., Valiente N., Cheng H., Latorre A., Nishida C., Ghosn E.E.B., Kobayashi M. Frontiers in Immunology. 2022;13

    • Ding J., Cardoso A.A., Yoshimoto M., Kobayashi M. The Earliest T-Precursors in the Mouse Embryo Are Susceptible to Leukemic Transformation Frontiers in Cell and Developmental Biology. 2021;9

    • Portilho N.A., Scarfo R., Bertesago E., Ismailoglu I., Kyba M., Kobayashi M., Ditadi A., Yoshimoto M. Development (Cambridge). 2021;148(15)

    • Yu H., Gao R., Chen S., Liu X., Wang Q., Cai W., Vemula S., Fahey A.C., Henley D., Kobayashi M., Liu S.Z., Qian Z., Kapur R., Broxmeyer H.E., Gao Z., Xi R., Liu Y. Stem Cell Reviews and Reports. 2021;17:2304-2313.

    • Chen S., Wang Q., Yu H., Capitano M.L., Vemula S., Nabinger S.C., Gao R., Yao C., Kobayashi M., Geng Z., Fahey A., Henley D., Liu S.Z., Barajas S., Cai W., Wolf E.R., Ramdas B., Cai Z., Gao H., Luo N., Sun Y., Wong T.N., Link D.C., Liu Y., Boswell H.S., Mayo L.D., Huang G., Kapur R., Yoder M.C., Broxmeyer H.E., Gao Z., Liu Y. Nature Communications. 2020;11(1)

    • Kobayashi M., Lin Y., Mishra A., Shelly C., Gao R., Reeh C.W., Wang P.Z., Xi R., Liu Y., Wenzel P., Ghosn E., Liu Y., Yoshimoto M. Journal of Immunology. 2020;204(12):3262-3272.

    • Kobayashi M., Yoshimoto M. A Modified Semisolid Clonal Culture for Identification of B-1 and B-2 Progenitor Colony Forming Ability of Mouse Embryonic Hemogenic Endothelial Cells Bio-protocol. 2020;10(9)

    • Azevedo Portilho N., Kobayashi M., Yoshimoto M. International Journal of Hematology. 2019;109(1):35-40.

    • Chen S., Wang Q., Yu H., Capitano M.L., Vemula S., Nabinger S.C., Gao R., Yao C., Kobayashi M., Geng Z., Fahey A., Henley D., Liu S.Z., Barajas S., Cai W., Wolf E.R., Ramdas B., Cai Z., Gao H., Luo N., Sun Y., Wong T.N., Link D.C., Liu Y., Boswell H.S., Mayo L.D., Huang G., Kapur R., Yoder M.C., Broxmeyer H.E., Gao Z., Liu Y. Nature Communications. 2019;10(1)

    • Kobayashi M., Tarnawsky S.P., Wei H., Mishra A., Azevedo Portilho N., Wenzel P., Davis B., Wu J., Hadland B., Yoshimoto M. Stem Cell Reports. 2019;13(1):21-30.

    • Lin Y., Kobayashi M., Azevedo Portilho N., Mishra A., Gao H., Liu Y., Wenzel P., Davis B., Yoder M.C., Yoshimoto M. Stem Cell Reports. 2019;12(3):572-583.

    • Nabinger S., Chen S., Gao R., Yao C., Kobayashi M., Vemula S., Fahey A., Wang C., Daniels C., Boswell H., Sandusky G., Mayo L., Kapur R., Liu Y. Leukemia. 2019;33(6):1535-1539.

    • Chen S., Gao R., Yao C., Kobayashi M., Liu S.Z., Yoder M.C., Broxmeyer H., Kapur R., Boswell H.S., Mayo L.D., Liu Y. Leukemia. 2018;32(3):850-854.

    • Spidale N.A., Sylvia K., Narayan K., Miu B., Frascoli M., Melichar H.J., Zhihao W., Kisielow J., Palin A., Serwold T., Love P., Kobayashi M., Yoshimoto M., Jain N., Kang J. Immunity. 2018;49(5):857-872.e5.

    • Chen S., Gao R., Kobayashi M., Yu H., Yao C., Kapur R., Yoder M., Liu Y. Experimental Hematology. 2017;45:74-84.

    • Kobayashi M., Bai Y., Chen S., Gao R., Yao C., Cai W., Cardoso A.A., Croop J., Zhang Z.Y., Liu Y. Leukemia. 2017;31(3):751-754.

    • Kobayashi M., Chen S., Bai Y., Yao C., Gao R., Sun X., Mu C., Twiggs T., Yu Z., Boswell H., Yoder M., Kapur R., Mulloy J., Zhang Z., Liu Y. Leukemia. 2017;31(6):1453-1457.

    • Kobayashi M., Nabinger S.c., Bai Y., Yoshimoto M., Gao R., Chen S., Yao C., Dong Y., Zhang L., Rodriguez S., Yashiro-Ohtani Y., Pear W.S., Carlesso N., Yoder M.C., Kapur R., Kaplan M.H., Daniel Lacorazza H., Zhang Z.Y., Liu Y. Stem Cells. 2017;35(4):1053-1064.

    • Yao C., Kobayashi M., Chen S., Nabinger S.C., Gao R., Liu S.Z., Asai T., Liu Y. Oncotarget. 2017;8(50):87607-87622.

    • Ghosh J., Kobayashi M., Ramdas B., Chatterjee A., Ma P., Mali R.S., Carlesso N., Liu Y., Plas D.R., Chan R.J., Kapur R. Journal of Clinical Investigation. 2016;126(7):2621-2625.

    • Gao R., Chen S., Kobayashi M., Yu H., Zhang Y., Wan Y., Young S.K., Soltis A., Yu M., Vemula S., Fraenkel E., Cantor A., Antipin Y., Xu Y., Yoder M.C., Wek R.C., Ellis S.R., Kapur R., Zhu X., Liu Y. Stem Cells. 2015;33(3):925-938.

    • Chatterjee A., Ghosh J., Ramdas B., Mali R.S., Martin H., Kobayashi M., Vemula S., Canela V.H., Waskow E.R., Visconte V., Tiu R.V., Smith C.C., Shah N., Bunting K.D., Boswell H.S., Liu Y., Chan R.J., Kapur R. Cell Reports. 2014;9(4):1333-1348.

    • Chitteti B.R., Kobayashi M., Cheng Y., Zhang H., Poteat B.A., Broxmeyer H.E., Pelus L.M., Hanenberg H., Zollman A., Kamocka M.M., Carlesso N., Cardoso A.A., Kacena M.A., Srour E.F. Blood. 2014;124(4):519-529.

    • Kobayashi M., Bai Y., Dong Y., Yu H., Chen S., Gao R., Zhang L., Yoder M., Kapur R., Zhang Z., Liu Y. Stem Cells. 2014;32(7):1956-1967.

    • Kobayashi M., Chen S., Gao R., Bai Y., Zhang Z., Liu Y. Cell Cycle. 2014;13(18):2827-2835.

    • Kobayashi M., Shelley W.C., Seo W., Vemula S., Lin Y., Liu Y., Kapur R., Taniuchi I., Yoshimoto M. Proceedings of the National Academy of Sciences of the United States of America. 2014;111(33):12151-12156.

    • Kobayashi M., Srour E.F. Blood. 2011;118(25):6562-6571.

    • Hiramatsu H., Morishima T., Nakanishi H., Mizushima Y., Miyazaki M., Matsubara H., Kobayashi M., Nakahata T., Adachi S. Bone Marrow Transplantation. 2008;41(10):907-908.

    • Matsubara H., Kobayashi M., Yui Y., Kato I., Niwa A., Arai M., Miyazaki M., Mizushima Y., Umeda K., Hiramatsu H., Watanabe K.i., Adachi S., Nakahata T. [Rinsh艒 ketsueki] The Japanese journal of clinical hematology. 2007;48(8):605-610.

    • Niwa A., Matsubara H., Adachi S., Fujino H., Higashi Y., Umeda K., Shiota M., Hiramatsu H., Kobayashi M., Watanabe K.I., Yorifuji T., Nakahata T. Pediatrics International. 2007;49(4):530-532.

    • Yoshihara T., Okada K., Kobayashi M., Kikuta A., Kato K., Adachi N., Kikuchi A., Ishida H., Hirota Y., Kuroda H., Nagatoshi Y., Inukai T., Koike K., Kigasawa H., Yagasaki H., Tokuda K., Kishimoto T., Nakano T., Fujita N., Goto H., Nakazawa Y., Kanegane H., Matsuzaki A., Osugi Y., Hasegawa D., Uoshima N., Nakamura K., Tsuchida M., Tanaka R., Watanabe A., Yabe H. International Journal of Hematology. 2007;85(3):246-255.

    • Matsubara H., Kobayashi M., Tokumasu M., Nakanishi H., Miyazaki M., Mizushima Y., Hiramatsu H., Adachi S., Nakayama T., Onishi E., Nakahata T. Leukemia and Lymphoma. 2006;47(7):1435-1437.

    • Mizumoto H., Hata D., Yamamoto K., Shirakawa R., Kumakura A., Shiota M., Yokoyama A., Matsubara H., Kobayashi M., Nishikomori R., Adachi S., Nakahata T., Kita T., Horiuchi H., Yasukawa M., Ishii E. European Journal of Pediatrics. 2006;165(6):384-388.

    • Yamamoto A., Miki Y., Adachi S., Kanagaki M., Fushimi Y., Okada T., Kobayashi M., Hiramatsu H., Umeda K., Nakahata T., Van Buchem M.A., Togashi K. European Journal of Radiology. 2006;57(3):423-427.

    • Kasahara M., Ueda M., Haga H., Hiramatsu H., Kobayashi M., Adachi S., Sakamoto S., Oike F., Egawa H., Takada Y., Tanaka K. American Journal of Transplantation. 2005;5(9):2229-2235.

    • Matsubara H., Adachi S., Yano J., Kitamura N., Miyazaki M., Mizushima Y., Hiramatsu H., Kobayashi M., Nakahata T. International Journal of Hematology. 2004;80(2):190-192.

    • Matsubara H., Watanabe K.i., Sakai H., Chang H., Fujino H., Higashi Y., Kobayashi M., Adachi S., Seto S., Nakahata T. Spine. 2004;29(1)

    • Tanaka T., Matsubara H., Adachi S., Chang H., Fujino H., Higashi Y., Yasumi T., Kobayashi M., Watanabe K.I., Takahashi M., Kobayashi Y., Maruya E., Saji H., Nakahata T. International Journal of Hematology. 2004;80(5):467-469.

    • Toyoda H., Ido M., Hori H., Hiraiwa H., Hirayama M., Kobayashi M., Ogawa M., Miyahara M., Iwamoto S., Hayashi T., Gabazza E.C., Suzuki K., Azuma E., Komada Y. Journal of Pediatric Hematology/Oncology. 2004;26(9):606-608.

    • Iwamoto S., Azuma E., Hori H., Hirayama M., Kobayashi M., Komada Y., Nishimori H., Miyahara M., Nishide Y. Pediatric Hematology and Oncology. 2002;19(4):255-261.

    • Azuma E., Hirayama M., Bonno M., Iwamoto S., Kumamoto T., Kobayashi M., Komada Y., Taniguchi K., Nakano T., Kamiya H. Pediatric Hematology and Oncology. 2001;18(3):193-197.

    • Jiang Q., Azuma E., Hirayama M., Iwamoto S., Kumamoto T., Kobayashi M., Yamamoto H., Sakurai M., Komada Y. Pediatrics International. 2001;43(4):334-339.

    • Jiang Q., Azuma E., Tanaka M., Kobayashi M., Hirayama M., Kumamoto T., Iwamoto S., Yamamoto H., Nakashima K., Sakurai M., Komada Y. Clinical and Experimental Immunology. 2001;125(2):222-228.

    • Kobayashi M., Azuma E., Ido M., Hirayama M., Jiang Q., Iwamoto S., Kumamoto T., Yamamoto H., Sakurai M., Komada Y. Journal of Immunology. 2001;167(7):3585-3591.

    • Toyoda H., Azuma E., Hori H., Hirayama M., Kobayashi M., Isogai K., Kondo N., Komada Y. Bone Marrow Transplantation. 2001;28(4):413-415.

    • Hirayama M., Azuma E., Jiang Q., Kobayashi M., Iwamoto S., Kumamoto T., Kisenge R., Yamamoto H., Komada Y. British Journal of Haematology. 2000;111(2):659-661.

    • Hirayama M., Azuma E., Jiang Q., Kobayashi M., Iwamoto S., Kumamoto T., Kisenge R., Yamamoto H., Komada Y. British Journal of Haematology. 2000;111(2):700-707.

    • Miyahara M., Azuma E., Hirayama M., Kobayashi M., Hori H., Komada Y., Masuda H. Pediatric Hematology and Oncology. 2000;17(6):489-495.

    • Kumamoto T., Azuma E., Tanaka M., Qi J., Hirayama M., Zhang S.L., Kobayashi M., Iwamoto S., Komada Y., Yamamoto H., Nakashima K., Sakurai M. British Journal of Haematology. 1999;105(4):1025-1033.

    • Hirayama M., Azuma E., Kumamoto T., Qi J., Kobayashi M., Komada Y., Inui T., Miyake M., Mugishima H., Hamazaki M., Sakurai M. Bone Marrow Transplantation. 1998;22(9):923-926.

    • Umemoto M., Azuma E., Hirayama M., Nagai M., Hiratake S., Qi J., Kumamoto T., Kobayashi M., Komada Y., Sakurai M. Clinical and Experimental Immunology. 1998;112(3):459-463.